Seminar - Tom Cech

photo of Dr. Tom Cech
March 27, 2019 - 12:30pm
Location: 
Davis Auditorium

Distinguished Professor

Director of BioFrontiers Institute

Investigator, HHMI

University of Colorado, Boulder

Host: Jim Manley

Title: RNA-Protein Interactions Regulate Epigenetic Silencing and Cardiomyocyte Differentiation

Abstract: Many nuclear DNA-binding proteins also bind RNA, often inexplicably. For example, the histone methyltransferase Polycomb Repressive Complex 2 (PRC2) binds nuclear RNAs with high affinity and limited specificity, but it has been highly controversial whether these interactions are functionally important. We have used CRISPR-Cas9 genome editing to introduce an RNA-binding-defective mutant PRC2 into human induced pluripotent stem cells (iPSCs) and find that the mutant iPSCs have a striking defect in cardiomyocyte differentiation. Genome-wide analyses reveal how RNA binding is essential for maintaining epigenetic repression.

Business Office

Department of Biological Sciences
500 Fairchild Center
Mail Code 2401
Columbia University
1212 Amsterdam Avenue
New York, NY 10027

Academic Office

Department of Biological Sciences
600 Fairchild Center
Mail Code 2402
Columbia University
1212 Amsterdam Avenue
New York, NY 10027
biology@columbia.edu
212 854-4581