Andreas Beyer, Ph.D.
Professor, Institute for Genetics, University of Cologne, Germany
Host: Harmen Bussemaker
Title: The Impact of Genomic Variability on Protein States and Regulatory Networks
Genomic variation affects cellular networks by altering molecular abundances (such as RNA and protein levels) and by affecting molecular states such as protein phosphorylation. However, previous work has mostly focused on the former, thereby largely neglecting genomic effects on regulatory networks. In order to systematically study the impact of genomic variability on protein biosynthesis and regulatory networks, we generated high-quality transcriptome, proteome, and phosphoproteome data for a panel of 112 genetically diverse yeast strains. We found a significant uncoupling of the transcript-protein relationship for certain protein classes, such as subunits of protein complexes. Integration with the phosphoproteomics data suggested that the same genetic locus often affected distinct genes at the level of protein biosynthesis and phosphorylation. The number of affected phospho-traits was most predictive for cellular growth effects, thus underlining the importance of effects on regulatory networks for understanding genotype-phenotype relationships. This study shows how multi-layered molecular networks mediate the effects of genomic variants to more complex physiological traits and highlights the particular importance of protein phosphorylation.