BIOL
G4260
Proteomics Laboratory
Semester: 
Fall 2015
Section Number: 
001
Days of the Week: 
Thursdays
Time: 
9:00am - 12:15pm
Number of Credits: 
3
Course Description: 

Starting with fall 2009, this course will now be offered only in the fall semester. Open to students in M.A. in Biotechnology Program (points can be counted against laboratory requirement for that program), Ph.D. and advanced undergraduate students with background in genetics or molecular biology. Students should be comfortable with basic biotechnology laboratory techniques as well as being interested in doing computational work in a Windows environment. This course deals with the proteome: the expressed protein complement of a cell, matrix, tissue, organ or organism. The study of the proteome (proteomics) is broadly applicable to life sciences research, and is increasing important in academic, government and industrial research through extension of the impact of advances in genomics. These techniques are being applied to basic research, exploratory studies of cancer and other diseases, drug discovery and many other topics. Techniques of protein extraction, two-dimensional gel electrophoresis and mass spectrometry will be covered. Emphasis will be on mastery of practical techniques of MALDI-TOF mass spectrometry and database searching for identification of proteins separated by gel electrophoresis as well as background tutorials and exercises covering other techniques used in descriptive and comparative proteomics. Lab Fee: $150.

Business Office

Department of Biological Sciences
500 Fairchild Center
Mail Code 2401
Columbia University
1212 Amsterdam Avenue
New York, NY 10027

Academic Office

Department of Biological Sciences
600 Fairchild Center
Mail Code 2402
Columbia University
1212 Amsterdam Avenue
New York, NY 10027
biology@columbia.edu
212 854-4581