University of California, San Francisco
School of Medicine
Host: Molly Przeworski
Title: Regenerating Stentor: Single cell regeneration comes of age
Abstract: Throughout development, many organisms lose the ability to regenerate new tissue following injury. Organisms that retain regenerative ability are powerful model systems and have uncovered mechanisms of regeneration, particularly the role of cell-to-cell signaling. Yet little is known about regeneration at the level of an individual cell. The giant ciliate Stentor coeruleus has remarkable regenerative capabilities: when removed by excision, nearly any portion of the cell will give rise to a normally proportioned cell with intact subcellular organization. While pioneering studies of Stentor elucidated the morphological principles of its regeneration, molecular principles remain unknown. We have recently published the draft genome of Stentor coeruleus (Current Biology, 2017). This invaluable tool opens the door to uncovering the molecular details underlying Stentor’s regeneration and re-patterning programs. We have also analyzed dynamic changes in Stentor’s transcriptome during regeneration with high temporal resolution (Bioarxiv, 2018). Our findings suggest that the precise morphological stages of regeneration are driven by a complex regulatory system. This work establishes the basis for understanding the principles of single-cell regeneration.